Estimation of dna by diphenylamine method pdf

In DNA, only the deoxyribose of purine nucleotide reacts so that the value obtained represents one half of the total deoxyribose produced. Materials Commercial sample of DNA — 10 mg. Buffer saline 0. Diphenyl amine reagent: dissolved in 10 gms of pure diphenyl amine in 1 liter of glacial acetic acid and add 25 mL of concentrated H 2 SO 4. This solution must be prepared fresh. Anal Lett 19 5—6 — Lecault V, Patel N, Thibault J An image analysis technique to estimate the cell density and biomass concentration of Trichoderma reesei.

Lett Appl Microbiol 48 4 — Lehninger AL Biochemistry. Worth, New York. Biotechnol Bioeng 47 5 — J Appl Microbiol 4 — Part IX. Some properties and reactions of 2-deoxy- d -galactose. Appl Environ Microbiol 55 4 — Gene 61 2 — J Bacteriol 22 — R Development Core Team R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Sambrook J, Russell D Molecular cloning: a laboratory manual. Mol Microbiol 66 2 — Schaffeld G, Illanes A Determination of cell mass in an insoluble lignocellulosic substrate in submerged fermentation.

Biotechnol Lett 4 10 — Schmidt G, Thannhauser SJ A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. J Biol Chem — Schneider WC Phosphorus compounds in animal tissues: extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid.

Schneider WC Phosphorus compounds in animal tissues: a comparison of methods for the estimation of nucleic acids. J Biol Chem 2 — Computation from kinetics of deoxyribose extraction in hot acid. Acta Chem Scand B 37 4 — Microbiology 2 — J Microbiol Methods 88 2 — Appl Microbiol Biotechnol 96 5 — Metab Eng 11 4—5 — Download references. This work is supported by the Young Scientists Fund grant no. Materials » DNA Standard 0. Procedure 1. Prepare separate marked tubes containing 1mL, 2mL and 3mL aliquots of the isolated DNA dissolved in standard saline citrate and similar aliquots of a 0.

Make all sample tubes, and a separate blank, up to 3mL with H 2 O. Add 6mL of diphenylamine reagent to each tube, and after mixing, heat the tubes in a boiling water bath for 10 min.